Abstract
Molecular surveillance of Chikungunya virus (CHIKV) is important as it provides data on the circulating CHIKV genotypes in endemic countries and enabling activation of measures to be taken in the event of a pending outbreak. Molecular surveillance is carried out by first detecting CHIKV in susceptible humans or among field-caught mosquitoes. This is followed by sequencing a selected region of the virus which will provide evidence on the source of the virus and possible association of the virus to increased cases of Chikungunya infections.
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References
Singh RK, Tiwari S, Mishra VK, Tiwari R, Dhole TN (2012) Molecular epidemiology of Chikungunya virus: mutation in E1 gene region. J Virol Methods 185:213–220
Powers AM, Brault AC, Tesh RB, Weaver SC (2000) Re-emergence of Chikungunya and O’nyong-nyong viruses: evidence for distinct geographical lineages and distinct evolutionary relationships. J Gen Virol 81:471–479
Schuffenecker I, Iteman I, Michault A, Murri Frangeul S, Vaney L et al (2006) Genome microevolution of Chikungunya viruses causing the Indian Ocean outbreak. PloS Med 3, e263
Powers AM, Logue CH (2007) Changing patterns of Chikungunya virus: re emergence of a zoonotic arbovirus. J Gen Virol 88:2363–2377
Laksmipathy DT, Dhanasekaran D (2008) Molecular epidemiology of Chikungunya virus in Vellore District, Tamilnadu, India in 2006. East Afr J Public Health 5(2):122–125
Chem YK, Zainah S, Beremdam SJ, Tengku Rogayah TAR, Khairul AH, Chua KB (2010) Molecular epidemiology of Chikungunya virus in Malaysia since its first emergence in 1998. Med J Malaysia 5(1):31–35
Noridah O, Paranthaman V, Nayar SK, Masliza M, Ranjit K, Norizah I, Chem YK, Mustafa B, Kumarasamy V, Chua KB (2007) Outbreak of Chikungunya due to virus of Central/East African Genotype 1 in Malaysia. Med J Malaysia 62(4):323–328
Bortel WV, Dorleans F, Rosine J, Blateau A, Rousset D, Matheus S, Leparc-Goffart I, Flusin O et al (2014) Chikungunya outbreak in the Caribbean region, December 2013 to March 2014, and the significance for Europe. Euro Surveill 19(13):pii: 20759
Laurent P, Roux KL, Grivard P, Bertil G, Naze F, Picard M, Staikowsky F, Barau G, Schuffenecker I, Michault A (2007) Development of a sensitive real time reverse transcriptase PCR assay with an internal control to detect and quantify Chikungunya virus. Clin Chem 53(8):1408–1414
Santhosh SR, Dash PL, Parida MM, Khan M, Tiwari M, Lakshmana Rao PV (2008) Comparative full genome analysis revealed E1: A226V shift in 2007 Indian Chikungunya virus isolates. Virus Res 135:36–41
Rianthavorn P, Prianantathavorn K, Wuttirattanakowit N, Theamboonlers A, Poovorawan Y (2010) An outbreak of Chikungunya in southern Thailand from 2008-2009 caused by African strains with A226V mutation. Int J Infect Dis 145:161–165
Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poorawan Y (2012) Multiplex real time Rt-PCR for detecting Chikungunya virus and dengue virus. Asian Pac J Trop Dis 5(5):342–346
Apandi YM, Lau SK, Norfaezah A, Nur Izmawati AR, Liyana AZ, Khairul Izwan H, Zainah S (2011) Epidemiology and molecular characterization of Chikungunya virus involved in the 2008 and 2009 outbreak in Malaysia. J Gen Mol Virol 3(2):35–42
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
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Thayan, R., Yusof, M.A., Saat, Z., Sekaran, S.D., Wang, S.M. (2016). Molecular Epidemiology of Chikungunya Virus by Sequencing. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_2
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DOI: https://doi.org/10.1007/978-1-4939-3618-2_2
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