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Viral–Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

Yeast two-hybrid (Y2H) assay is one of the earliest methods developed to study protein–protein interactions. In the proteomics era, Y2H has created a niche of its own by providing protein interaction maps for various organisms. Owing to limited coding capacities of their genomes, viruses are dependent on their host cellular machinery for successful infection. Identification of the key players orchestrating the survival of virus in their host is essential for understanding viral life cycle and devising strategies to prevent interactions resulting in pathogenesis. In this chapter, Y2H assay will be explained in detail for studying viral–host protein interactions of Chikungunya virus (CHIKV).

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Author information

Authors and Affiliations

  1. Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307, Uttar Pradesh, India

    Namrata Dudha & Sanjay Gupta

Authors
  1. Namrata Dudha
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  2. Sanjay Gupta
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Corresponding author

Correspondence to Sanjay Gupta .

Editor information

Editors and Affiliations

  1. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Swee Kim Ang

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© 2016 Springer Science+Business Media New York

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Dudha, N., Gupta, S. (2016). Viral–Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

  • eBook Packages: Springer Protocols

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