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Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

Having a mechanism to assess the transmission dynamics of a vector-borne virus is one critical component of understanding the life cycle of these viruses. Laboratory infection systems using artificial blood meals is one valuable approach for monitoring the progress of virus in its mosquito host and evaluating potential points for interruption of the cycle for control purposes. Here, we describe an artificial blood meal system with Chikungunya virus (CHIKV) and the processing of mosquito tissues and saliva to understand the movement and time course of virus infection in the invertebrate host.

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Author information

Authors and Affiliations

  1. Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO, 80521, USA

    Jeremy P. Ledermann & Ann M. Powers

Authors
  1. Jeremy P. Ledermann
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  2. Ann M. Powers
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Corresponding author

Correspondence to Ann M. Powers .

Editor information

Editors and Affiliations

  1. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Swee Kim Ang

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© 2016 Springer Science+Business Media New York

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Ledermann, J.P., Powers, A.M. (2016). Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

  • eBook Packages: Springer Protocols

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