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Protein Quantitation of the Developing Cochlea Using Mass Spectrometry

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Auditory and Vestibular Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1427))

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Abstract

Mass spectrometry-based proteomics allows for the measurement of hundreds to thousands of proteins in a biological system. Additionally, mass spectrometry can also be used to quantify proteins and peptides. However, observing quantitative differences between biological systems using mass spectrometry-based proteomics can be challenging because it is critical to have a method that is fast, reproducible, and accurate. Therefore, to study differential protein expression in biological samples labeling or label-free quantitative methods can be used. Labeling methods have been widely used in quantitative proteomics, however label-free methods have become equally as popular and more preferred because they produce faster, cleaner, and simpler results. Here, we describe the methods by which proteins are isolated and identified from cochlear sensory epithelia tissues at different ages and quantitatively differentiated using label-free mass spectrometry.

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Acknowledgements

This work was supported by the NIH/NIDCD Grant R01 DC004295 to BHAS.

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Authors and Affiliations

  1. Department of Otolaryngology—HNS, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL, 33612, USA

    Lancia N. F. Darville & Bernd H. A. Sokolowski

Authors
  1. Lancia N. F. Darville
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  2. Bernd H. A. Sokolowski
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Corresponding author

Correspondence to Lancia N. F. Darville .

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Editors and Affiliations

  1. College of Medicine, University of South Florida, Tampa, Florida, USA

    Bernd Sokolowski

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© 2016 Springer Science+Business Media New York

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Darville, L.N.F., Sokolowski, B.H.A. (2016). Protein Quantitation of the Developing Cochlea Using Mass Spectrometry. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology, vol 1427. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3615-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-3615-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3613-7

  • Online ISBN: 978-1-4939-3615-1

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