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Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes

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Book cover Dendritic Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1423))

Abstract

Dendritic cells (DCs) are a rare population of antigen-presenting cells that initiate immune responses in secondary lymphoid organs. In order to better understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.

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References

  1. Villadangos JA, Schnorrer P (2007) Intrinsic and cooperative antigen-presenting functions of dendritic-cell subsets in vivo. Nat Rev Immunol 7(7):543–555

    Article  CAS  PubMed  Google Scholar 

  2. McIlroy D, Troadec C, Grassi F, Samri A, Barrou B, Autran B, Debre P, Feuillard J, Hosmalin A (2001) Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors. Blood 97(11):3470–3477

    Article  CAS  PubMed  Google Scholar 

  3. Lindstedt M, Lundberg K, Borrebaeck CA (2005) Gene family clustering identifies functionally associated subsets of human in vivo blood and tonsillar dendritic cells. J Immunol 175(8):4839–4846

    Article  CAS  PubMed  Google Scholar 

  4. Segura E, Durand M, Amigorena S (2013) Similar antigen cross-presentation capacity and phagocytic functions in all freshly isolated human lymphoid organ-resident dendritic cells. J Exp Med 210(5):1035–1047

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S (2012) Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 209(4):653–660

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Villadangos JA, Heath WR (2005) Life cycle, migration and antigen presenting functions of spleen and lymph node dendritic cells: limitations of the Langerhans cells paradigm. Semin Immunol 17(4):262–272

    Article  CAS  PubMed  Google Scholar 

  7. Haniffa M, Shin A, Bigley V, McGovern N, Teo P, See P, Wasan PS, Wang XN, Malinarich F, Malleret B, Larbi A, Tan P, Zhao H, Poidinger M, Pagan S, Cookson S, Dickinson R, Dimmick I, Jarrett RF, Renia L, Tam J, Song C, Connolly J, Chan JK, Gehring A, Bertoletti A, Collin M, Ginhoux F (2012) Human tissues contain CD141(hi) cross-presenting dendritic cells with functional homology to mouse CD103(+) nonlymphoid dendritic cells. Immunity 37(1):60–73

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Duluc D, Gannevat J, Anguiano E, Zurawski S, Carley M, Boreham M, Stecher J, Dullaers M, Banchereau J, Oh S (2013) Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol 6(3):626–638

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Elodie Segura .

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© 2016 Springer Science+Business Media New York

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Durand, M., Segura, E. (2016). Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes. In: Segura, E., Onai, N. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 1423. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3606-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3606-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3604-5

  • Online ISBN: 978-1-4939-3606-9

  • eBook Packages: Springer Protocols

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