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Dendritic Cell Protocols pp 269–274Cite as

In Vivo Imaging of Cutaneous DCs in Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1423))

Abstract

Varieties of cells orchestrate immune responses. To capture such dynamic phenomena, intravital imaging is an important technique, and it may provide substantial information that is not available using conventional histological analyses. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of biological samples with high spatiotemporal resolution, and it has now become the leading method for in vivo imaging studies. Here we describe a basic method for in vivo imaging of dendritic cells (DCs) in the mouse ear skin using multiphoton microscopy.

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Dermatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara, Kyoto, 606-8507, Japan

    Gyohei Egawa & Kenji Kabashima M.D., Ph.D.

  2. PRESTO, Japan science and Technology Agency, Saitama, 332-0012, Japan

    Kenji Kabashima M.D., Ph.D.

  3. Singapore Immunology Network (SIgN) and Institute of MedicalBiology, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore

    Kenji Kabashima M.D., Ph.D.

Authors
  1. Gyohei Egawa
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  2. Kenji Kabashima M.D., Ph.D.
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Corresponding author

Correspondence to Kenji Kabashima M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Pavillon Pasteur, Institut Curie INSERM U932, Paris Cedex 05, France

    Elodie Segura

  2. Department of Biodefense, Tokyo Medical and Dental University, Tokyo, Japan

    Nobuyuki Onai

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Egawa, G., Kabashima, K. (2016). In Vivo Imaging of Cutaneous DCs in Mice. In: Segura, E., Onai, N. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 1423. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3606-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-3606-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3604-5

  • Online ISBN: 978-1-4939-3606-9

  • eBook Packages: Springer Protocols

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