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Gastrointestinal Physiology and Diseases pp 89–99Cite as

Analysis of microRNA Levels in Intestinal Epithelial Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1422))

Abstract

The field of microRNA (miRNA) research is expanding rapidly with the crucial role of miRNAs in almost every biological process and their implication in many diseases. The role of miRNAs in modulating inflammatory responses in the gut has attracted many research groups including us. Here, we first briefly summarize our current understanding of the role of miRNAs in maintaining and regulating gut physiopathology and in inflammatory bowel diseases. We then describe in detail our techniques to analyze miRNA levels with notes that we have collected and summarized during our experiments.

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Author information

Authors and Affiliations

  1. UMR 1071 Inserm, University of Auvergne, 28 Place Henri Dunant, Clermont-Ferrand, 63001, France

    Hang Thi Thu Nguyen Ph.D.

Authors
  1. Hang Thi Thu Nguyen Ph.D.
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Corresponding author

Correspondence to Hang Thi Thu Nguyen Ph.D. .

Editor information

Editors and Affiliations

  1. Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, Virginia, USA

    Andrei I. Ivanov

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© 2016 Springer Science+Business Media New York

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Nguyen, H.T.T. (2016). Analysis of microRNA Levels in Intestinal Epithelial Cells. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3603-8_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3601-4

  • Online ISBN: 978-1-4939-3603-8

  • eBook Packages: Springer Protocols

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