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Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells

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Gastrointestinal Physiology and Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1422))

Abstract

Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

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Acknowledgments

This work was supported by NIH 1R01DK083402 grant (Zavros), NIH 5T32GM105526 grant (Bertaux-Skeirik), and the Albert J. Ryan Fellowship (Bertaux-Skeirik).

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Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA

    Nina Bertaux-Skeirik, Jomaris Centeno & Yana Zavros Ph.D.

  2. Department of Gastroenterology and Hepatology, The First Affiliated Hospital, Sun Yatsen University, Guangzhou, China

    Rui Feng

  3. Division of Gastroenterology, Children’s Hospital Los Angeles, Los Angeles, CA, USA

    Michael A. Schumacher

  4. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA

    Ramesh A. Shivdasani

  5. Department of Medicine, Harvard Medical School, Boston, MA, USA

    Ramesh A. Shivdasani

Authors
  1. Nina Bertaux-Skeirik
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  2. Jomaris Centeno
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  3. Rui Feng
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  4. Michael A. Schumacher
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  5. Ramesh A. Shivdasani
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  6. Yana Zavros Ph.D.
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Corresponding author

Correspondence to Yana Zavros Ph.D. .

Editor information

Editors and Affiliations

  1. Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, Virginia, USA

    Andrei I. Ivanov

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© 2016 Springer Science+Business Media New York

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Bertaux-Skeirik, N., Centeno, J., Feng, R., Schumacher, M.A., Shivdasani, R.A., Zavros, Y. (2016). Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3603-8_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3601-4

  • Online ISBN: 978-1-4939-3603-8

  • eBook Packages: Springer Protocols

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