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Macrophage Isolation from the Mouse Small and Large Intestine

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Gastrointestinal Physiology and Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1422))

Abstract

Macrophages play important roles in maintaining intestinal homeostasis via their ability to orchestrate responses to the normal microbiota as well as pathogens. One of the most important steps in beginning to understand the functions of these cells is the ability to effectively isolate them from the complex intestinal environment. Here, we detail methodology for the isolation and phenotypic characterization of macrophages from the mouse small and large intestine.

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Abbreviations

APC:

Antigen-presenting cells

DC:

Dendritic cell

LI:

Large intestine

LP:

Lamina propria

SI:

Small intestine

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Acknowledgements

This work was supported by National Institutes of Health Grants 1R01DK097256 (to T.L.D.) and 1F30DK097904-03 (to D.G.).

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Authors and Affiliations

  1. Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, 30303, USA

    Akihito Harusato

  2. Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA

    Duke Geem

  3. Institute for Biomedical Sciences, Georgia State University, 100 Piedmont Avenue, Room 690, Atlanta, GA, 30303, USA

    Timothy L. Denning

Authors
  1. Akihito Harusato
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  2. Duke Geem
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  3. Timothy L. Denning
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Corresponding author

Correspondence to Timothy L. Denning .

Editor information

Editors and Affiliations

  1. Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, Virginia, USA

    Andrei I. Ivanov

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Harusato, A., Geem, D., Denning, T.L. (2016). Macrophage Isolation from the Mouse Small and Large Intestine. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3603-8_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3601-4

  • Online ISBN: 978-1-4939-3603-8

  • eBook Packages: Springer Protocols

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