Abstract
Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitis-associated colon cancer.
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Gounaris, E., Ishihara, Y., Shrivastrav, M., Bentrem, D., Barrett, T.A. (2016). Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_13
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DOI: https://doi.org/10.1007/978-1-4939-3603-8_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3601-4
Online ISBN: 978-1-4939-3603-8
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