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Plant Nitric Oxide pp 175–189Cite as

Detection of S-Nitrosoglutathione Reductase Activity in Plants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1424))

Abstract

S-nitrosoglutathione reductase (GSNOR) is considered a key enzyme in the regulation of intracellular levels of S-nitrosoglutathione and protein S-nitrosylation. As a part of nitric oxide catabolism, GSNOR catalyzes the irreversible decomposition of GSNO to oxidized glutathione. GSNOR is involved in the regulation of plant growth and development, mediated by NO-dependent signaling mechanisms, and is known to play important roles in plant responses to various abiotic and biotic stress conditions. Here we present optimized protocols to determine GSNOR enzyme activities in plant samples by spectrophotometric measurements and by activity staining after the native polyacrylamide gel electrophoresis.

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Acknowledgments

This project was supported by the grants Kontakt LH11013 and GAČR P501/12/0590. We thank Prof. Juan B. Barroso (Universidad of Jaén, Spain) and Dr. Javier F. Corpas (CSIC Granada, Spain), for valuable collaboration and sharing practical knowledge in the initial steps of our research in plant NO and GSNOR field.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Palacky University, Šlechtitelů 11, 78371, Olomouc, Czech Republic

    Lucie Kubienová, Tereza Tichá, Lenka Luhová & Marek Petřivalský

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  1. Lucie Kubienová
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  2. Tereza Tichá
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  3. Lenka Luhová
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  4. Marek Petřivalský
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Corresponding author

Correspondence to Marek Petřivalský .

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Editors and Affiliations

  1. National Institute of Plant Genome Resea, New Delhi, India

    Kapuganti Jagadis Gupta

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Kubienová, L., Tichá, T., Luhová, L., Petřivalský, M. (2016). Detection of S-Nitrosoglutathione Reductase Activity in Plants. In: Gupta, K. (eds) Plant Nitric Oxide. Methods in Molecular Biology, vol 1424. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3600-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3600-7_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3598-7

  • Online ISBN: 978-1-4939-3600-7

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