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Forensic DNA Typing Protocols pp 201–211Cite as

Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1420))

Abstract

Rapidly mutating (RM) Y-STRs were recently identified and can help to differentiate between paternally related males in paternity and forensic casework. Normal Y-STRs are often used in casework due to their paternal inheritance, which can help to resolve kinship cases, and identify male components in male/female mixtures; however, more discriminating profiles are obtained if rapidly mutating Y-STRs are used. Previously two or three multiplex PCRs have been used to amplify 13 RM Y-STRs; here, an assay amplifying these 13 markers in a single multiplex PCR is described. Commercially available male control DNA samples have been genotyped during the validation of this assay, thus providing a tool for calibrating genotyping results. It is expected that the assay will provide a niche tool for genotyping casework samples.

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Author information

Authors and Affiliations

  1. School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK

    Sibte Hadi

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  1. Sibte Hadi
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Correspondence to Sibte Hadi .

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Editors and Affiliations

  1. School of Forensic and Applied Sciences, University of Central Lancashire, Preston, Lancashire, United Kingdom

    William Goodwin

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Hadi, S. (2016). Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs). In: Goodwin, W. (eds) Forensic DNA Typing Protocols. Methods in Molecular Biology, vol 1420. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3597-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3597-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3595-6

  • Online ISBN: 978-1-4939-3597-0

  • eBook Packages: Springer Protocols

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