Abstract
Characterizing protein–protein and protein–RNA interaction networks is a fundamental step to understanding the function of an RNA-binding protein. In many cases, these interactions are transient and highly dynamic. Therefore, capturing stable as well as transient interactions in living cells for the identification of protein-binding partners and the mapping of RNA-binding sequences is key to a successful establishment of the molecular interaction network. In this chapter, we will describe a method for capturing the molecular interactions in living cells using formaldehyde as a crosslinker and enriching a specific RNA–protein complex from cell extracts followed by mass spectrometry and Next-Gen sequencing analyses.
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Yeh, HS., Chang, JW., Yong, J. (2016). Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks. In: Lin, RJ. (eds) RNA-Protein Complexes and Interactions. Methods in Molecular Biology, vol 1421. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3591-8_14
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DOI: https://doi.org/10.1007/978-1-4939-3591-8_14
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