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A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells

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Apoptosis Methods in Toxicology

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Apoptosis, or programmed cell death, is a tightly regulated cellular event that plays an important role in both normal developmental processes and many pathological states. The induction of apoptosis is tightly regulated through the coordinated action of members of the caspase family of proteases. Here we discuss a relatively inexpensive protocol for monitoring the induction and progression of apoptosis using a genetically encoded fluorescence resonance energy transfer (FRET)-based biosensor of the executioner caspase, caspase-3, in living suspension cells.

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Author information

Authors and Affiliations

  1. Department of Energy and Environmental Studies, North Carolina A&T State University, Greensboro, NC, 27411, USA

    Akamu J. Ewunkem

  2. Department of Biology, North Carolina A&T State University, 1601 East Market Street, Greensboro, NC, 27411, USA

    Carl D. Parson II, Perpetua M. Muganda & Robert H. Newman

Authors
  1. Akamu J. Ewunkem
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  2. Carl D. Parson II
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  3. Perpetua M. Muganda
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  4. Robert H. Newman
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Corresponding author

Correspondence to Robert H. Newman .

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Editors and Affiliations

  1. Department of Biology, North Carolina A&T State University, Greensboro, North Carolina, USA

    Perpetua M. Muganda

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Ewunkem, A.J., Parson, C.D., Muganda, P.M., Newman, R.H. (2016). A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells. In: Muganda, P. (eds) Apoptosis Methods in Toxicology. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3588-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3588-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3586-4

  • Online ISBN: 978-1-4939-3588-8

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