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Novel Electrochemical Biosensor for Apoptosis Evaluation

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Book cover Apoptosis Methods in Toxicology

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

Apoptosis evaluation is one of the most important tasks of toxicology. By using a peptide as the recognition element, and assembling apoptotic cells on a solid surface, we have established a novel electrochemical method for the detection of apoptosis levels. Such a peptide-based electrochemical biosensor is simple, cost-effective, convenient, and sensitive. Since the results obtained are well in line with other standard methods, this method holds a great potential towards the analysis of apoptosis and its applications. In this chapter, we introduce a general overview of this technical approach for detecting apoptotic cells. We discuss its advantages over the ordinary methods. We also provide practical guidelines for designing studies, and summarize the step-by-step protocols used in our lab for sample preparation, electrode modification, and accurate electrochemical quantification of apoptotic cells.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 21307154 and 31400847) and the National Key Instrument Developing Project of China (Grant No. ZDYZ2013-1).

Author information

Authors and Affiliations

  1. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Road, Suzhou, Jiangsu, 215163, China

    Peng Miao & Jian Yin

Authors
  1. Peng Miao
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  2. Jian Yin
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Corresponding author

Correspondence to Peng Miao .

Editor information

Editors and Affiliations

  1. Department of Biology, North Carolina A&T State University, Greensboro, North Carolina, USA

    Perpetua M. Muganda

Glossary

CV

Cyclic voltammetry

DPV

Differential pulse voltammetry

ECL

Electrochemiluminescent

EDTA

Ethylenediaminetetraacetic acid

EIS

Electrochemical impedance spectroscopy

MCH

Mercaptohexanol

PBS

Phosphate buffered saline

PI

Propidium iodide

TCEP

Tris(2-carboxyethyl)phosphinehydro-chloride

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Miao, P., Yin, J. (2016). Novel Electrochemical Biosensor for Apoptosis Evaluation. In: Muganda, P. (eds) Apoptosis Methods in Toxicology. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3588-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3588-8_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3586-4

  • Online ISBN: 978-1-4939-3588-8

  • eBook Packages: Springer Protocols

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