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Mesenchymal Stem Cells pp 425–441Cite as

Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1416))

Abstract

Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.

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Acknowledgments

The authors thank Prof. Takehisa Matsuda of the Kyoto Institute of Technology (Japan) for assistance with the synthesis of styrenated gelatins. This work was supported by a Grant-in-Aid for Scientific Research (24300173, 24120007), a Management Expenses Grant for National Universities Corporations (Nano-Macro Materials, Devices and System Research Alliance) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

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Authors and Affiliations

  1. Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan

    Thasaneeya Kuboki & Satoru Kidoaki

Authors
  1. Thasaneeya Kuboki
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  2. Satoru Kidoaki
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Corresponding author

Correspondence to Satoru Kidoaki .

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Editors and Affiliations

  1. Dept of Molecular Med,Unit of Cardiology, University of Pavia, PAVIA, Italy

    Massimiliano Gnecchi

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Kuboki, T., Kidoaki, S. (2016). Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture. In: Gnecchi, M. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology, vol 1416. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3584-0_25

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  • DOI: https://doi.org/10.1007/978-1-4939-3584-0_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3582-6

  • Online ISBN: 978-1-4939-3584-0

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