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Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection

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Programmed Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1419))

Abstract

Gene delivery methods are important for both therapeutic intervention and as tools in research to address specific questions. Hydrodynamic injection (HDI) is a method that facilitates the delivery and expression of genetic material in target cells, namely hepatocytes, through an intravenous injection. HDI has great utility for research involving cell death and signaling pathways essential in the processes of cancer, inflammation, and transplant therapy, as well as representing a valuable technique to establish hepatitis B virus (HBV) expression in hepatocytes. This chapter describes in detail how to generate a model of chronic HBV infection in immunocompetent mice using HDI as a delivery method.

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Acknowledgments

We thank Pei-Jer Chen and Ding-Shin Chen for constructing the HBV encoding plasmid for hydrodynamic injection.

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Authors and Affiliations

  1. The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia

    Simon P. Preston, Marc Pellegrini & Gregor Ebert

  2. Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia

    Simon P. Preston, Marc Pellegrini & Gregor Ebert

Authors
  1. Simon P. Preston
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  2. Marc Pellegrini
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  3. Gregor Ebert
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Corresponding author

Correspondence to Gregor Ebert .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Inst for Mol Sci, La Trobe Univ, Bundoora, Victoria, Australia

    Hamsa Puthalakath

  2. Department of Biochemistry and Genetics, La Trobe Inst for Mol Sci, La Trobe Univ, Bundoora, Victoria, Australia

    Christine J. Hawkins

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Preston, S.P., Pellegrini, M., Ebert, G. (2016). Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection. In: Puthalakath, H., Hawkins, C. (eds) Programmed Cell Death. Methods in Molecular Biology, vol 1419. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3581-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3581-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3579-6

  • Online ISBN: 978-1-4939-3581-9

  • eBook Packages: Springer Protocols

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