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NLR Proteins pp 89–95Cite as

Measuring IL-1β Processing by Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1417))

Abstract

IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and in living macrophages either using a plate reader or a microscope.

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References

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Acknowledgments

This work was supported by the European Research Council and Instituto de Salud Carlos III-FEDER grants to P.P. V.C. was supported by the Institut National de la Santé et de la Recherche Médicale.

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Authors and Affiliations

  1. Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France

    Vincent Compan

  2. Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France

    Vincent Compan

  3. Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Carretera Buenavista, s/n, Murcia, 30120, Spain

    Pablo Pelegrín

Authors
  1. Vincent Compan
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  2. Pablo Pelegrín
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Corresponding author

Correspondence to Pablo Pelegrín .

Editor information

Editors and Affiliations

  1. Department of Morphology Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy

    Francesco Di Virgilio

  2. Muricia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca Murcia, Molecular Inflammation Group, Murcia, Spain

    Pablo Pelegrín

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Compan, V., Pelegrín, P. (2016). Measuring IL-1β Processing by Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor. In: Di Virgilio, F., Pelegrín, P. (eds) NLR Proteins. Methods in Molecular Biology, vol 1417. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3566-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3566-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3564-2

  • Online ISBN: 978-1-4939-3566-6

  • eBook Packages: Springer Protocols

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