Abstract
Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here, we describe a method to detect polyubiquitination of NRLP3 using two different approaches: (1) detection with an ubiquin antibody or (2) using TUBE (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLR or other components of the inflammasome.
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Acknowledgements
This work was supported by funds from the Manchester Collaborative Centre for Inflammation Research and a Sir Henry Dale fellowship from the Wellcome Trust (UK).
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Palazón-Riquelme, P., López-Castejón, G. (2016). Method to Measure Ubiquitination of NLRs. In: Di Virgilio, F., Pelegrín, P. (eds) NLR Proteins. Methods in Molecular Biology, vol 1417. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3566-6_16
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DOI: https://doi.org/10.1007/978-1-4939-3566-6_16
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3566-6
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