Abstract
Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Martinon F, Burns K, Tschopp J (2002) The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 10(2):417–426
Mariathasan S, Weiss DS, Newton K, McBride J, O’Rourke K, Roose-Girma M, Lee WP, Weinrauch Y, Monack DM, Dixit VM (2006) Cryopyrin activates the inflammasome in response to toxins and ATP. Nature 440(7081):228–232. doi:10.1038/nature04515
Compan V, Baroja-Mazo A, Lopez-Castejon G, Gomez AI, Martinez CM, Angosto D, Montero MT, Herranz AS, Bazan E, Reimers D, Mulero V, Pelegrin P (2012) Cell volume regulation modulates NLRP3 inflammasome activation. Immunity 37(3):487–500. doi:10.1016/j.immuni.2012.06.013
Guarda G, Zenger M, Yazdi AS, Schroder K, Ferrero I, Menu P, Tardivel A, Mattmann C, Tschopp J (2011) Differential expression of NLRP3 among hematopoietic cells. J Immunol 186(4):2529–2534. doi:10.4049/jimmunol.1002720
Compan V, Baroja-Mazo A, Bragg L, Verkhratsky A, Perroy J, Pelegrin P (2012) A genetically encoded IL-1beta bioluminescence resonance energy transfer sensor to monitor inflammasome activity. J Immunol 189(5):2131–2137. doi:10.4049/jimmunol.1201349
Acknowledgments
This work was supported by funds from the Manchester Collaborative Centre for Inflammation Research and a Sir Henry Dale fellowship from the Wellcome Trust (UK) to G.L.-C. V.C. was supported by the Institut National de la Santé et de la Recherche Médicale.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Compan, V., López-Castejón, G. (2016). Functional Reconstruction of NLRs in HEK293 Cells. In: Di Virgilio, F., Pelegrín, P. (eds) NLR Proteins. Methods in Molecular Biology, vol 1417. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3566-6_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3566-6_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3564-2
Online ISBN: 978-1-4939-3566-6
eBook Packages: Springer Protocols