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Mass Spectrometry-Based Proteomics in Biomarker Discovery for Neurodegenerative Diseases

  • Sravani Musunuri
  • Ganna Shevchenko
  • Jonas BergquistEmail author
Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Several neurodegenerative disorders such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), Machado–Joseph disease (MJD), and amyotrophic lateral sclerosis (ALS) lead to disease-specific changes in the neuronal proteins. The usage of rapidly evolving proteomic technologies, such as mass spectrometry (MS), has opened new avenues to detect the changes in the protein expression in disease vs. control samples for understanding biochemical pathogenesis in neurodegenerative disorders. Efficient sample preparation is an integral part of a successful MS-based proteomics. Apart from the identification, quantification of the proteins is needed to investigate the alterations between proteome profiles from different sample sets. This chapter provides an overview of the sample collection, preparation, identification, and quantification of proteins using MS in the biomarker discovery for the neurodegenerative diseases.

Key words

Alzheimer’s disease Biological samples Mass spectrometry Neuroproteomics Parkinson’s disease Protein purification Sample preparation Separation techniques Qualitative analysis Quantitative analysis 

Notes

Acknowledgments

This research was supported by Uppsala Berzelii Technology Centre for Neurodiagnostics, with financing from the Swedish Governmental Agency for Innovation Systems and The Swedish Research Council 621-2011-4423. We acknowledge Dr. Denys Shevchenko for technical assistance with Fig. 4.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sravani Musunuri
    • 1
  • Ganna Shevchenko
    • 1
  • Jonas Bergquist
    • 1
    Email author
  1. 1.Analytical Chemistry, Department of Chemistry-BMCSciLifeLab Uppsala UniversityUppsalaSweden

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