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Oxygenation of Anandamide by Lipoxygenases

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1412))

Abstract

The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase, monoacylglycerol lipase, and α,β-hydrolases 6 and 12, but they also serve as substrates for cyclooxygenases and lipoxygenases. These enzymes oxygenate the 1Z,4Z-pentadiene system of the arachidonic acid backbone of endocannabinoids, thereby giving rise to an entirely new array of bioactive lipids. Hereby, a protocol is provided for the enzymatic synthesis, purification, and characterization of various oxygenated metabolites of anandamide generated by lipoxygenases, which enables the biological study and detection of these metabolites.

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Author information

Authors and Affiliations

  1. Cellular Protein Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Guus van Zadelhoff

  2. Department Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands

    Mario van der Stelt

Authors
  1. Guus van Zadelhoff
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  2. Mario van der Stelt
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Corresponding author

Correspondence to Mario van der Stelt .

Editor information

Editors and Affiliations

  1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

    Mauro Maccarrone

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van Zadelhoff, G., van der Stelt, M. (2016). Oxygenation of Anandamide by Lipoxygenases. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3539-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3537-6

  • Online ISBN: 978-1-4939-3539-0

  • eBook Packages: Springer Protocols

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