Abstract
Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.
Key words
- Superresolution microscopy (SRM)
- Structured illumination microscopy (SIM)
- Single-molecule localization microscopy (SMLM)
- Stochastic optical reconstruction microscopy (STORM)
- Photoactivated localization microscopy (PALM)
- Nuclear envelope (NE)
- Nuclear lamins
- Nuclear pore complex (NPC)
- Synaptonemal complex
- Linker of the nucleoskeleton and cytoskeleton (LINC) complex
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Acknowledgments
Srivats Hariharan (Olympus Singapore, formerly IMB, A*STAR) for building the SMLM microscope in the IMB Microscopy Unit together with the lab of Sohail Ahmed (IMB, A*STAR), John Lim Soon Yew (IMB, A*STAR) for assisting with the maintenance and operation of the SMLM microscope and for supporting the image processing and analysis, Declan Lunny (IMB, A*STAR) for the advice and help with the sample preparation, and Brian Burke (IMB, A*STAR) and Colin Stewart (IMB, A*STAR) for supervising the research projects within their labs.
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Xie, W., Horn, H.F., Wright, G.D. (2016). Superresolution Microscopy of the Nuclear Envelope and Associated Proteins. In: Shackleton, S., Collas, P., Schirmer, E. (eds) The Nuclear Envelope. Methods in Molecular Biology, vol 1411. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3530-7_4
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DOI: https://doi.org/10.1007/978-1-4939-3530-7_4
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