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Label-Free Quantitation for Clinical Proteomics

  • Robert Moulder
  • Young Ah Goo
  • David R. GoodlettEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1410)

Abstract

Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS-based label-free quantitation using standard data-dependent acquisition.

Key words

Label-free quantification Proteomics Mass spectrometry Area under the curve (AUC) 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Robert Moulder
    • 1
  • Young Ah Goo
    • 2
  • David R. Goodlett
    • 2
    Email author
  1. 1.Centre for BiotechnologyUniversity of TurkuTurkuFinland
  2. 2.Mass Spectrometry CenterUniversity of Maryland—BaltimoreBaltimoreUSA

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