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Development of a Targeted Gene-Delivery System Using Escherichia coli

  • Chung-Jen Chiang
  • Chih-Hsiang Chang
  • Yun-Peng Chao
  • Ming-Ching Kao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1409)

Abstract

A gene-delivery system based on microbes is useful for development of targeted gene therapy of non-phagocytic cancer cells. Here, the feasibility of the delivery system is illustrated by targeted delivery of a transgene (i.e., eukaryotic GFP) by Escherichia coli to HER2/neu-positive cancer cells. An E. coli strain was engineered with surface display of the anti-HER2/neu affibody. To release the gene cargo, a programmed lysis system based on phage ϕX174 gene E was introduced into the E. coli strain. As a result, 3 % of HER2/neu-positive cells that were infected with engineered E. coli were able to express the GFP.

Key words

Bacterial carrier Targeted delivery HER2/neu Bactofection 

Notes

Acknowledgments

This work is supported by Feng Chia University (08G27501), National Science Council of Taiwan (NSC101-2221-E-035-0575-MY3, NSC102-2622-E-035- 005-CC1), and China Medical University (CMU98-C-11).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Medical Laboratory Science and BiotechnologyChina Medical UniversityTaichungTaiwan
  2. 2.Department of Chemical EngineeringFeng Chia UniversityTaichungTaiwan
  3. 3.Department of Biological Science and TechnologyChina Medical UniversityTaichungTaiwan

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