Development of a Targeted Gene-Delivery System Using Escherichia coli
A gene-delivery system based on microbes is useful for development of targeted gene therapy of non-phagocytic cancer cells. Here, the feasibility of the delivery system is illustrated by targeted delivery of a transgene (i.e., eukaryotic GFP) by Escherichia coli to HER2/neu-positive cancer cells. An E. coli strain was engineered with surface display of the anti-HER2/neu affibody. To release the gene cargo, a programmed lysis system based on phage ϕX174 gene E was introduced into the E. coli strain. As a result, 3 % of HER2/neu-positive cells that were infected with engineered E. coli were able to express the GFP.
Key wordsBacterial carrier Targeted delivery HER2/neu Bactofection
This work is supported by Feng Chia University (08G27501), National Science Council of Taiwan (NSC101-2221-E-035-0575-MY3, NSC102-2622-E-035- 005-CC1), and China Medical University (CMU98-C-11).
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