Oral Delivery of Tumor-Targeting Salmonella to Treat Cancer in Mice
Tumor-targeting bacteria have been developed as novel anticancer agents recently. To achieve their therapeutic effects, bacteria have conventionally been injected intravenously or intraperitoneally into animals or humans. Here, we describe the oral delivery of tumor-targeting Salmonella for cancer therapy in a mouse tumor model. We detail the experimental procedures for establishing a mouse tumor model, preparing bacterial culture, mouse gavage, and detection of the tumor-targeting capability of bacteria administered orally. We also discuss technical notes and provide practical advice that will help the users of this oral delivery model.
Key wordsOral delivery Tumor-targeting bacteria Salmonella Solid tumor Cancer therapy
We thank Lihui Li and Xiaoxin Zhang for their technical assistance. This study was supported by grants from the Chinese National Natural Science Foundation (30500637 to Lijun Jia; 81472793 to Dongping Wei) and Key Project of the Shanghai Municipal Health Bureau (2010012 to Lijun Jia).
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