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Methods for Tumor Targeting with Salmonella typhimurium A1-R

  • Robert M. Hoffman
  • Ming Zhao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1409)

Abstract

Salmonella typhimurium A1-R (S. typhimurium A1-R) has shown great preclinical promise as a broad-based anti-cancer therapeutic (please see Chapter  1). The present chapter describes materials and methods for the preclinical study of S. typhimurium A1-R in clinically-relevant mouse models. Establishment of orthotopic metastatic mouse models of the major cancer types is described, as well as other useful models, for efficacy studies of S. typhimurium A1-R or other tumor-targeting bacteria, as well. Imaging methods are described to visualize GFP-labeled S. typhimurium A1-R, as well as GFP- and/or RFP-labeled cancer cells in vitro and in vivo, which S. typhimurium A1-R targets. The mouse models include metastasis to major organs that are life-threatening to cancer patients including the liver, lung, bone, and brain and how to target these metastases with S. typhimurium A1-R. Various routes of administration of S. typhimurium A1-R are described with the advantages and disadvantages of each. Basic experiments to determine toxic effects of S. typhimurium A1-R are also described. Also described are methodologies for combining S. typhimurium A1-R and chemotherapy. The testing of S. typhimurium A1-R on patient tumors in patient-derived orthotopic xenograft (PDOX) mouse models is also described. The major methodologies described in this chapter should be translatable for clinical studies.

Key words

Salmonella typhimurium A1-R Tumor targeting Leu-Arg Amino acid Auxotroph Green fluorescent protein (GFP) Surgical orthotopic implantation (SOI) Nude mice Mouse models Cancer Metastasis Orthotopic 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.AntiCancer Inc. and Department of SurgeryUniversity of California – San DiegoSan DiegoUSA
  2. 2.AntiCancer Inc.San DiegoUSA

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