Abstract
Optogenetics is emerging in the cardiology field as a new strategy to explore biological functions through the use of light-sensitive proteins and dedicated light sources. For example, this technology allows modification of the electrophysiological properties of cardiac muscle cells with superb spatiotemporal resolution and quantitative control. In this chapter, the optogenetic modification of atrial cardiomyocytes (aCMCs) from 2-day-old Wistar rats using lentiviral vector (LV) technology and the subsequent activation of the light-sensitive proteins (i.e., ion channels) through light-emitting diodes (LEDs) are described.
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Acknowledgement
This work was supported by a VIDI grant (91714336) from the Dutch Organization for Scientific Research (NWO) to Daniël Pijnappels. Antoine de Vries is a recipient of a Chinese Exchange Programme grant (10CDP007) from the Royal Netherlands Academy of Arts and Sciences (KNAW) and received additional support by ICIN-Netherlands Heart Institute.
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Feola, I., Teplenin, A., de Vries, A.A.F., Pijnappels, D.A. (2016). Optogenetic Engineering of Atrial Cardiomyocytes. In: Kianianmomeni, A. (eds) Optogenetics. Methods in Molecular Biology, vol 1408. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3512-3_22
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DOI: https://doi.org/10.1007/978-1-4939-3512-3_22
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