Abstract
Increasing concern regarding the use of animals in research has triggered a growing need for non-animal research models in a range of fields. The development of 3Rs (replacement, refinement, and reduction) approaches in research, to reduce the reliance on the use of animal tissue and whole-animal experiments, has recently included the use of Dictyostelium. In addition to not feeling pain and thus being relatively free of ethical constraints, Dictyostelium provides a range of distinct methodological advantages for researchers that has led to a number of breakthroughs. These methodologies include using cell behavior (cell movement and shape) as a rapid indicator of sensitivity to poorly characterized medicines, natural products, and other chemicals to help understand the molecular mechanism of action of compounds. Here, we outline a general approach to employing Dictyostelium as a 3Rs research model, using cell behavior as a readout to better understand how compounds, such as the active ingredient in chilli peppers, capsaicin, function at a cellular level. This chapter helps scientists unfamiliar with Dictyostelium to rapidly employ it as an advantageous model system for research, to reduce the use of animals in research, and to make paradigm shift advances in our understanding of biological chemistry.
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Acknowledgements
M.C. is funded by GlaxoSmithKline. G.P.O. is funded by the Dr. Hadwen Trust (DHT) and did not participate in experiments involving animals, or cells or tissues from animals or from human embryos. The Dr. Hadwen Trust is the UK leading medical research charity that funds and promotes exclusively human-relevant research that encourages the progress of medicine with the replacement of the use of animals in research. Authors G.P.O. and M.C. contributed equally.
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Otto, G.P., Cocorocchio, M., Munoz, L., Tyson, R.A., Bretschneider, T., Williams, R.S.B. (2016). Employing Dictyostelium as an Advantageous 3Rs Model for Pharmacogenetic Research. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_9
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DOI: https://doi.org/10.1007/978-1-4939-3480-5_9
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