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Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells

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Chemotaxis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1407))

Abstract

Mitochondria not only play a critical and central role in providing metabolic energy to the cell but are also integral to the other cellular processes such as modulation of various signaling pathways. These pathways affect many aspects of cell physiology, including cell movement, growth, division, differentiation, and death. Mitochondrial dysfunction which affects mitochondrial bioenergetics and causes oxidative phosphorylation defects can thus lead to altered cellular physiology and manifest in disease. The assessment of the mitochondrial bioenergetics can thus provide valuable insights into the physiological state, and the alterations to the state of the cells. Here, we describe a method to successfully use the Seahorse XFe24 Extracellular Flux Analyzer to assess the mitochondrial respirometry of the cellular slime mold Dictyostelium discoideum.

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Acknowledgements

S. Lay and O. Sanislav contributed equally to this work, which was supported by the Australian Research Council Discovery Project grant DP140104276.

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Authors and Affiliations

  1. Discipline of Microbiology, La Trobe University, Bundoora, VIC, Australia

    Sui Lay & Oana Sanislav

  2. Department of Physiology Anatomy and Microbiology, Discipline of Microbiology, School of Life Sciences, College of Science Health and Engineering, La Trobe University, Melbourne, VIC, 3086, Australia

    Sarah J. Annesley & Paul R. Fisher

Authors
  1. Sui Lay
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  2. Oana Sanislav
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  3. Sarah J. Annesley
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  4. Paul R. Fisher
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Corresponding author

Correspondence to Paul R. Fisher .

Editor information

Editors and Affiliations

  1. Nat Inst of Allergy& Infectious Dis, NIH, Laboratory of Immunogenetics, Rockville, Maryland, USA

    Tian Jin

  2. National Institutes of Health, Nat Inst Alcohol Abuse & Alcoholism, Rockville, Maryland, USA

    Dale Hereld

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Lay, S., Sanislav, O., Annesley, S.J., Fisher, P.R. (2016). Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3480-5_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3478-2

  • Online ISBN: 978-1-4939-3480-5

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