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Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization

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Chemotaxis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1407))

Abstract

Since the first reports on chemokine function, much information has been generated on the implications of these molecules in numerous physiological and pathological processes, as well as on the signaling events activated through their binding to receptors. As is the case for other G protein-coupled receptors, chemokine receptors are not isolated entities that are activated following ligand binding; rather, they are found as dimers and/or higher order oligomers at the cell surface, even in the absence of ligands. These complexes form platforms that can be modified by receptor expression and ligand levels, indicating that they are dynamic structures. The analysis of the conformations adopted by these receptors at the membrane and their dynamics is thus crucial for a complete understanding of the function of the chemokines. We focus here on the methodology insights of new techniques, such as those based on resonance energy transfer for the analysis of chemokine receptor conformations in living cells.

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Acknowledgments

We specially thank the present and former members of the DIO chemokine group who contributed to some of the work described in this review. We also thank C. Bastos and C. Mark for secretarial support and editorial assistance, respectively. This work was partially supported by grants from the Spanish Ministry of Economy and Competitiveness (SAF-2011-27270), the RETICS Program (RD 12/0009/009 RIER) and the Madrid regional government (S2010/BMD-2350; RAPHYME).

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Authors and Affiliations

  1. Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Darwin 3 Campus de Cantoblanco, 28049, Madrid, Spain

    Laura Martínez-Muñoz, José Miguel Rodríguez-Frade & Mario Mellado

Authors
  1. Laura Martínez-Muñoz
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  2. José Miguel Rodríguez-Frade
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  3. Mario Mellado
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Corresponding author

Correspondence to Mario Mellado .

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Editors and Affiliations

  1. Nat Inst of Allergy& Infectious Dis, NIH, Laboratory of Immunogenetics, Rockville, Maryland, USA

    Tian Jin

  2. National Institutes of Health, Nat Inst Alcohol Abuse & Alcoholism, Rockville, Maryland, USA

    Dale Hereld

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Martínez-Muñoz, L., Rodríguez-Frade, J.M., Mellado, M. (2016). Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_24

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  • DOI: https://doi.org/10.1007/978-1-4939-3480-5_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3478-2

  • Online ISBN: 978-1-4939-3480-5

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