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Chemotaxis pp 217–228Cite as

Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1407))

Abstract

We describe three chemotaxis assays—Insall chambers, circular invasion assays, and 3D organotypic assays—that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.

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References

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Author information

Authors and Affiliations

  1. CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK

    Olivia Susanto, Max Nobis & Robert H. Insall

  2. Melanoma Clinic, Leeds Cancer Centre, St. James’s University Hospital, Beckett Street, Leeds, LS9 7TF, UK

    Andrew J. Muinonen-Martin

Authors
  1. Olivia Susanto
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  2. Andrew J. Muinonen-Martin
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  3. Max Nobis
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  4. Robert H. Insall
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Corresponding author

Correspondence to Robert H. Insall .

Editor information

Editors and Affiliations

  1. Nat Inst of Allergy& Infectious Dis, NIH, Laboratory of Immunogenetics, Rockville, Maryland, USA

    Tian Jin

  2. National Institutes of Health, Nat Inst Alcohol Abuse & Alcoholism, Rockville, Maryland, USA

    Dale Hereld

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© 2016 Springer Science+Business Media New York

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Susanto, O., Muinonen-Martin, A.J., Nobis, M., Insall, R.H. (2016). Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3480-5_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3478-2

  • Online ISBN: 978-1-4939-3480-5

  • eBook Packages: Springer Protocols

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