Abstract
In situ hybridization is an important technique in breast cancer research, which is widely applied in detection of specific nucleic acid sequences. Here, we describe the detailed protocol of fluorescence in situ hybridization and chromogenic in situ hybridization in detection of gene HER2/neu amplification in breast cancer tissues.
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References
John MSB (2004) Fluorescence in situ hybridization: technical overview. Molecular Diagnosis of Cancer Methods and Protocols, Second edition 1, 71–77
Wolfe KQ, Herrington CS (1997) Interphase cytogenetics and pathology: a tool for diagnosis and research. J Pathol 181:359–361
Bell SM, Zuo J, Myers RM et al (1996) Fluorescence in situ hybridization deletion mapping at 4p16.3 in bladder cancer cell lines refines the localisation of the critical interval to 30 kb. Genes Chromosomes Cancer 17:108–117
John MSB, Amanda F (2004) HER2 FISH in breast cancer. Molecular Diagnosis of Cancer Methods and Protocols, second edition 1, 89–103
Dale P, Jaana LD, Aparna K et al (2003) Molecular markers in ductal carcinoma in situ of the breast. Mol Cancer Res 1:362–375
Cole KD, He HJ, Wang L (2013) Breast cancer biomarker measurements and standards. Proteomics Clin Appl 7:17–29
Bartlett JMS, Going JJ, Mallon EA et al (2001) Evaluating HER2 amplification and overexpression in breast cancer. J Pathol 195:422–428
Young WK, Hee JL, Jong WL et al (2011) Dual-color silver-enhanced in situ hybridization for assessing HER2 gene amplification in breast cancer. Mod Pathol 24:794–800
Nitta H, Hauss-Wegrzyniak B, Lehrkamp M et al (2008) Development of automated brightfield double in situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (Cen 17) for breast carcinomas and an assay performance comparison to manual dual color HeR2 fluorescence in situ hybridization (FISH). Diagn Pathol 3:41
Ulla H, Sven M, Andreas S (2011) Dual color CISH and FISH to CISH conversion. IHC Staining Methods, Fifth edition 14, 97–101
Howlader N, Chen VW, Ries LA et al (2014) Overview of breast cancer collaborative stage data items-their definitions, quality, usage, and clinical implications: a review of SEER data for 2004–2010. Cancer 120(Suppl 23):3771–3780
Poulsen TS, Espersen ML, Kofoed V et al (2013) Comparison of fluorescence in situ hybridization and chromogenic in situ hybridization for low and high throughput HER2 genetic testing. Int J Breast Cancer 2013:368731
Iqbal N, Iqbal N (2014) Human epidermal growth factor receptor 2 (HER2) in cancers: overexpression and therapeutic implications. Mol Biol Int 2014:852748
Mitchell MS, Press MF (1999) The role of immunohistochemistry and fluorescence in situ hybridization for HER-2/neu in assessing the prognosis of breast cancer. Semin Oncol 26:108–116
Hanna WM, Rüschoff J, Bilous M et al (2014) HER2 in situ hybridization in breast cancer: clinical implications of polysomy 17 and genetic heterogeneity. Mod Pathol 27:4–18
Jorma I, Minna T (2004) Chromogenic in situ hybridization in tumor pathology. Molecular Diagnosis of Cancer Methods and Protocols, second edition 1, 133–144
Tanner M, Gancberg D, Di Leo A et al (2000) Chromogenic in situ hybridization (CISH): a practical new alternative for FISH in detection of HER-2/neu oncogene amplification. Am J Pathol 157:1467–1472
Jacquemier J, Spyratos F, Esterni B et al (2014) SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases. BMC Cancer 13:351
Shen EY (1969) Two-solution iron hematoxylin staining (Heidenhain) with HCl differentiation and tris-buffer neutralization. Stain Technol 44:209–210
Acknowledgement
This work was supported by National Nature Science Foundation of China (No. 81230046) and 973 Program of National Basic Research Program of China (No. 2015CB553906).
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Min, L., Shou, C. (2016). In Situ Hybridization of Breast Cancer Markers. In: Cao, J. (eds) Breast Cancer. Methods in Molecular Biology, vol 1406. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3444-7_4
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DOI: https://doi.org/10.1007/978-1-4939-3444-7_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3444-7
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