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Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression

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Breast Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1406))

Abstract

Proteolysis has been cited as an important contributor to cancer initiation and progression. One can take advantage of tumor-associated proteases to selectively deliver imaging agents. Protease-activated imaging systems have been developed using substrates designed for hydrolysis by members of the matrix metalloproteinase (MMP) family. We presently describe approaches by which one can optically image matrix metalloproteinase activity implicated in breast cancer progression, with consideration of selective versus broad protease probes.

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Acknowledgments

The methods described in this chapter reflect the pioneering work of the laboratories of Drs. Ralph Weissleder and W. Barry Edwards. We gratefully acknowledge the National Institutes of Health (EB000289 and CA098799) and the Texas Higher Education STAR Award Program for support of our laboratory’s research on matrix metalloproteinases.

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Authors and Affiliations

  1. Department of Chemistry & Biochemistry, Florida Atlantic University, Jupiter, FL, 33458, USA

    Gregg B. Fields & Maciej J. Stawikowski

  2. Department of Chemistry, The Scripps Research Institute/Scripps Florida, Jupiter, FL, 33458, USA

    Gregg B. Fields

  3. Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987, USA

    Gregg B. Fields

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  1. Gregg B. Fields
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  2. Maciej J. Stawikowski
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Correspondence to Gregg B. Fields .

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Editors and Affiliations

  1. School of Medicine, Stony Brook University, Stony Brook, New York, USA

    Jian Cao

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Fields, G.B., Stawikowski, M.J. (2016). Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression. In: Cao, J. (eds) Breast Cancer. Methods in Molecular Biology, vol 1406. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3444-7_25

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  • DOI: https://doi.org/10.1007/978-1-4939-3444-7_25

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