Abstract
Cancer cell invasion is a complex process that naturally occurs in a three-dimensional (3-D) environment comprised of tumor cells and extracellular matrix components (ECM). Therefore, examining the invasive ability of breast cancer cells in a 3-D assay is imperative to discovering novel treatment strategies aimed at preventing cancer invasion and metastasis. Here, I describe a method to quantitatively measure the number of invaded cancer cells within a 3-D microenvironment and determine the effects of potential drugs on this cellular process.
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Evensen, N.A. (2016). Three-Dimensional Assay for Studying Breast Cancer Cell Invasion. In: Cao, J. (eds) Breast Cancer. Methods in Molecular Biology, vol 1406. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3444-7_16
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DOI: https://doi.org/10.1007/978-1-4939-3444-7_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3442-3
Online ISBN: 978-1-4939-3444-7
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