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Dendrites: Recording from Fine Neuronal Structures Using Patch-Clamp and Imaging Techniques

  • Sonia GaspariniEmail author
  • Lucy M. Palmer
Protocol
Part of the Neuromethods book series (NM, volume 113)

Abstract

Dendrites are the principal site of synaptic input onto neurons but despite their importance in neuronal signaling, little is known about how they receive and transform this input. This is due largely to their typically submicron size, which has historically rendered them inaccessible for direct recording. However, the advent of electrophysiological patch-clamp and advanced imaging techniques over the past few decades has opened this field of research. Fuelled by Rall’s theory of active dendritic integration, intracellular recording techniques proved that dendrites do indeed have active conductances which modify synaptic input and thereby alter neuronal output in response to certain patterns of information. Furthermore, advances in fluorescence imaging have highlighted the importance of dendritic activity during sensory processing and behavior. Here we summarize advances in experimental methods, namely electrophysiological and fluorescence imaging techniques, which have improved the accessibility of recording from fine dendritic structures.

Key words

Dendrite Imaging Synapse Dendritic channels In vitro Brain slice Calcium indicators GFP 

Notes

Acknowledgements

This work was supported by NIH grant NS069714 (to SG) and NHMRC grants 1063533 and 1085708 (to LMP).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Neuroscience Center of ExcellenceLSU Health Sciences CenterNew OrleansUSA
  2. 2.Florey Institute of Neuroscience and Mental HealthUniversity of MelbourneMelbourneAustralia

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