Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches

  • Mara Almog
  • Alon KorngreenEmail author
Part of the Neuromethods book series (NM, volume 113)


The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.

Key words

Nucleated patch Outside-out patch Voltage-gated channels Patch clamp Kinetic analysis Hodgkin-Huxley model 



This work was supported by a grant from the German-Israeli Foundation to AK (#1091-27.1/2010).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.The Mina and Everard Goodman Faculty of Life SciencesBar-Ilan UniversityRamat-GanIsrael
  2. 2.Leslie and Susan Gonda Multidisciplinary Brain Research CenterBar-Ilan UniversityRamat-GanIsrael

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