Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches
The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.
Key wordsNucleated patch Outside-out patch Voltage-gated channels Patch clamp Kinetic analysis Hodgkin-Huxley model
This work was supported by a grant from the German-Israeli Foundation to AK (#1091-27.1/2010).
- 5.Johnston D, Wu SM-s (1995) Foundations of cellular neurophysiology. MIT Press, Cambridge, MAGoogle Scholar
- 20.Lin YC, Liu YC, Huang YY, Lien CC (2010) High-density expression of Ca2+-permeable ASIC1a channels in NG2 glia of rat hippocampus. PLoS One 5(9)Google Scholar