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ChIP-Seq: Library Preparation and Sequencing

  • Karyn L. Sheaffer
  • Jonathan SchugEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1402)

Abstract

Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-Seq) has been used extensively to determine the genome-wide location of DNA-binding factors, such as transcription factors, posttranscriptionally modified histones, and members of the transcription complex, to assess regulatory input, epigenetic modifications, and transcriptional activity, respectively. Here we describe methods to isolate chromatin from tissues, immunoprecipitate DNA bound to a protein of interest, and perform next-generation sequencing to identify a genome-wide DNA-binding pattern.

Key words

Chromatin immunoprecipitation Next-generation sequencing DNA binding 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Genetics, and Institute for Diabetes, Obesity and Metabolism, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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