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Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray

  • Xinna ZhangEmail author
  • Gabriel Lopez-Berestein
  • Anil K. Sood
  • George A. Calin
Part of the Methods in Molecular Biology book series (MIMB, volume 1402)

Abstract

Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. The abnormal expression of lncRNAs has been implicated in a range of many human diseases, including cancer. But only a small number of functional lncRNAs have been well characterized to date. lncRNA expression profiling may help to identify useful molecular biomarkers and targets for novel therapeutic approaches in the future. In this chapter, we describe a highly efficient lncRNA expression profiling method using a custom-designed microarray.

Key words

lncRNA expression Microarray profiling cDNA synthesis cDNA labeling 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Xinna Zhang
    • 1
    • 2
    Email author
  • Gabriel Lopez-Berestein
    • 7
    • 8
    • 10
  • Anil K. Sood
    • 4
    • 5
    • 6
  • George A. Calin
    • 3
    • 9
    • 11
  1. 1.Center for RNA Interference and Non-coding RNAsThe University of Texas MD Anderson Cancer CenterHoustonUSA
  2. 2.Department of Gynecologic OncologyThe University of Texas MD Anderson Cancer CenterHoustonUSA
  3. 3.Center for RNA Interference and Non-Coding RNAsThe University of Texas MD Anderson Cancer CenterHoustonUSA
  4. 4.Department of Gynecologic Oncology and Reproductive MedicineThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  5. 5.Center for RNA Interference and Non-Coding RNAsThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  6. 6.Department of Cancer BiologyThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  7. 7.Center for RNA Interference andNon-coding RNAsThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  8. 8.Department of Cancer BiologyThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  9. 9.Department of GynecologicOncology and ReproductiveMedicineThe University of Texas MD Anderson Cancer CenterHoustonUSA
  10. 10.Department of Cancer BiologyThe University of Texas MD Anderson Cancer Center (MDACC)HoustonUSA
  11. 11.Department of Experimental TherapeuticsThe University of Texas MD Anderson Cancer CenterHoustonUSA

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