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Long Non-Coding RNAs pp 215–227Cite as

Characterization of Circular RNAs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1402))

Abstract

Accumulated lines of evidence reveal that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5′ cap and 3′ tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus requiring specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs derived from intron lariats (ciRNAs) and circular RNAs produced from back spliced exons (circRNAs) in human.

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Acknowledgements

This work was supported by 31271390, 31322018, 91440202 from NSFC to L.Y. and L.L.C.

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Authors and Affiliations

  1. State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Yang Zhang & Ling-Ling Chen

  2. Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Li Yang

  3. School of Life Science and Technology, ShanghaiTech University, Shanghai, 200031, China

    Ling-Ling Chen

Authors
  1. Yang Zhang
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  2. Li Yang
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  3. Ling-Ling Chen
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Corresponding authors

Correspondence to Li Yang or Ling-Ling Chen .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Yi Feng

  2. School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Lin Zhang

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© 2016 Springer Science+Business Media New York

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Zhang, Y., Yang, L., Chen, LL. (2016). Characterization of Circular RNAs. In: Feng, Y., Zhang, L. (eds) Long Non-Coding RNAs. Methods in Molecular Biology, vol 1402. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3378-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3378-5_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3376-1

  • Online ISBN: 978-1-4939-3378-5

  • eBook Packages: Springer Protocols

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