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Non-isotopic Method for In Situ LncRNA Visualization and Quantitation

  • Botoul Maqsodi
  • Corina Nikoloff
Part of the Methods in Molecular Biology book series (MIMB, volume 1402)

Abstract

In mammals and other eukaryotes, most of the genome is transcribed in a developmentally regulated manner to produce large numbers of long noncoding RNAs (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and lncRNA) expression in a heterogeneous population of cells/tissues; however its utility has been hampered by complicated procedures typically developed and optimized for the detection of a specific gene and therefore not amenable to a wide variety of genes and tissues.

Recently, bDNA has revolutionized RNA in situ detection with fully optimized, robust assays for the detection of any mRNA and lncRNA targets in formalin-fixed paraffin-embedded (FFPE) and fresh frozen tissue sections using manual processing.

Key words

lncRNA in situ hybridization lncRNA visualization Branched DNA assay bDNA assay 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.AffymetrixSanta ClaraUSA

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