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Investigating the Endobacteria Which Thrive in Arbuscular Mycorrhizal Fungi

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Microbial Environmental Genomics (MEG)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1399))

Abstract

The study of the so-called unculturable bacteria is still considered a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, the identification and characterization of such microbes in complex biological samples is now possible. In this chapter we report how endobacteria thriving inside arbuscular mycorrhizal fungi (AMF), which are themselves obligate biotrophs of plants, can be studied using a combination of in vitro culture, molecular biology, and microscopy techniques.

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Acknowledgements

The authors wish to thank Mara Novero and Maria Teresa Della Beffa for having provided details on fungal culture conditions. Research in PB laboratory has been funded by the University of Turin (Local project 60 %).

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Authors and Affiliations

  1. Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, Turin, 48824, Italy

    Alessandro Desirò, Alessandra Salvioli & Paola Bonfante

  2. Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 10125, USA

    Alessandro Desirò

Authors
  1. Alessandro Desirò
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  2. Alessandra Salvioli
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  3. Paola Bonfante
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Corresponding author

Correspondence to Alessandro Desirò .

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Editors and Affiliations

  1. "Tree-Microbe Interactions" (IAM), UMR1136 INRA/University of Lorraine, Nancy, France

    Francis Martin

  2. "Tree-Microbe Interactions" (IAM);, UMR1136 INRA/University of Lorraine, Champenoux, France

    Stephane Uroz

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Desirò, A., Salvioli, A., Bonfante, P. (2016). Investigating the Endobacteria Which Thrive in Arbuscular Mycorrhizal Fungi. In: Martin, F., Uroz, S. (eds) Microbial Environmental Genomics (MEG). Methods in Molecular Biology, vol 1399. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3369-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3369-3_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3367-9

  • Online ISBN: 978-1-4939-3369-3

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