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Environmental Responses in Plants pp 357–372Cite as

Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1398))

Abstract

Nucleosome occupancy in promoter and genic regions can severely influence the transcription levels. Few methods have been established to investigate the nucleosome occupancy along the DNA. In this chapter we describe a detailed protocol to analyze the nucleosome occupancy at a specific locus using MNase-pPCR.

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Author information

Authors and Affiliations

  1. Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany

    Margaux Kaster & Sascha Laubinger

  2. Chemical Genomics Centre (CGC) of the Max Planck Society, 44227, Dortmund, Germany

    Margaux Kaster & Sascha Laubinger

  3. MPI for Developmental Biology, 72076, Tübingen, Germany

    Margaux Kaster & Sascha Laubinger

Authors
  1. Margaux Kaster
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  2. Sascha Laubinger
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Corresponding author

Correspondence to Sascha Laubinger .

Editor information

Editors and Affiliations

  1. Instituto Gulbenkian de Ciência, Oeiras, Portugal

    Paula Duque

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Kaster, M., Laubinger, S. (2016). Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR. In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_29

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  • DOI: https://doi.org/10.1007/978-1-4939-3356-3_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3354-9

  • Online ISBN: 978-1-4939-3356-3

  • eBook Packages: Springer Protocols

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