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Environmental Responses in Plants pp 293–308Cite as

Virus-Induced Gene Silencing for Gene Function Studies in Barley

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1398))

Abstract

Virus-Induced Gene Silencing (VIGS) creates a natural antiviral defense in plants. However, it has been also a powerful tool for endogenous gene silencing in dicot and monocot plants by exploitation of recombinant viruses, harboring silencing inducing sequences. The Barley Stripe Mosaic Virus (BSMV) based VIGS system is an efficient and rapid RNAi approach that is routinely applied in functional genomics studies of cereals. We present here a protocol for BSMV VIGS application in barley based on mechanical inoculation of the plants with in vitro transcribed recombinant BSMV RNAs as the silencing triggers.

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Acknowledgements

This work has been supported by the National Science Centre decision no DEC-2013/11/B/NZ9/01761. The pictures in Figs. 5 and 6 were taken by A. Pacak during his Marie Curie Intra-European fellowship BARPHO No. 025110, Aarhus University.

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  1. Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614, Poznań, Poland

    Maria Barciszewska-Pacak, Artur Jarmołowski & Andrzej Pacak

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  1. Maria Barciszewska-Pacak
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  2. Artur Jarmołowski
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  3. Andrzej Pacak
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Correspondence to Andrzej Pacak .

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  1. Instituto Gulbenkian de Ciência, Oeiras, Portugal

    Paula Duque

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Barciszewska-Pacak, M., Jarmołowski, A., Pacak, A. (2016). Virus-Induced Gene Silencing for Gene Function Studies in Barley. In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3356-3_22

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