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Environmental Responses in Plants pp 271–290Cite as

Construction of Artificial miRNAs to Prevent Drought Stress in Solanum tuberosum

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1398))

Abstract

The use of artificial microRNAs (amiRNAs) is still a relatively new technique in molecular biology with a wide range of applications in life sciences. Here, we describe the silencing of the CBP80/ABH1 gene in Solanum tuberosum with the use of amiRNA. The CBP80/ABH1 protein is part of the Cap Binding Complex (CBC), which is involved in plant responses to drought stress conditions. Transformed plants with a decreased level of CBP80/ABH1 display increased tolerance to water shortage conditions. We describe how to design amiRNA with the Web MicroRNA Designer platform in detail. Additionally, we explain how to perform all steps of a procedure aiming to obtain transgenic potato plants with the use of designed amiRNA, through callus tissue regeneration and Agrobacterium tumefaciens strain LBA4404 as a transgene carrier.

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Author notes

    Authors and Affiliations

    1. Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland

      Anna Wyrzykowska, Marcin Pieczynski & Zofia Szweykowska-Kulinska

    Authors
    1. Anna Wyrzykowska
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    2. Marcin Pieczynski
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    3. Zofia Szweykowska-Kulinska
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    Correspondence to Zofia Szweykowska-Kulinska .

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    Editors and Affiliations

    1. Instituto Gulbenkian de Ciência, Oeiras, Portugal

      Paula Duque

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    Wyrzykowska, A., Pieczynski, M., Szweykowska-Kulinska, Z. (2016). Construction of Artificial miRNAs to Prevent Drought Stress in Solanum tuberosum . In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_21

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    • DOI: https://doi.org/10.1007/978-1-4939-3356-3_21

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3354-9

    • Online ISBN: 978-1-4939-3356-3

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