Abstract
Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.
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Bennett KM, Zhou H, Sumner JP, Dodd SJ, Bouraoud N, Doi K, Star RA, Koretsky AP (2008) MRI of the basement membrane using charged nanoparticles as contrast agents. Magn Reson Med 60:564–574
Beeman SC, Zhang M, Gubhaju L, Wu T, Bertram JF, Frakes DH, Cherry BR, Bennett KM (2011) Measuring glomerular number and size in perfused kidneys using MRI. Am J Physiol Renal Physiol 300:F1454–F1457
Beeman SC, Cullen-McEwen LA, Puelles VG, Zhang M, Wu T, Baldelomar EJ, Dowling J, Charlton JR, Forbes MS, Ng A, Wu Q-Z, Armitage A, Egan GF, Bertram JF, Bennett KM (2014) MRI-based glomerular morphology and pathology in whole human kidneys. Am J Physiol Renal Physiol 306:F1381–F1390
Bennett KM, Bertram JF, Beeman SC, Gretz N (2013) The emerging role of MRI in quantitative renal glomerular morphology. Am J Physiol Renal Physiol 304:F1252–F1257
Charlton JR, Beeman JF, Bennett KM (2013) MRI-detectable nanoparticles: the potential role in the diagnosis of and therapy for chronic kidney disease. Adv Chronic Kidney Dis 20:479–487
Heilmann M, Neudecker S, Wolf I, Gubhaju L, Sticht C, Schock-Kusch D, Kruz W, Bertram JF, Schad LR, Gretz N (2012) Quantification of glomerular number and size distribution in normal rat kidneys using magnetic resonance imaging. Nephrol Dial Transplant 27:100–107
E. J. Baldelomar, J.R. Charlton, S.C. Beeman, L. Cullen-Mcewen, V.M. Pearl, J.F. Bertram, T. Wu, M. Zhang, K.M. Bennett. Phenotyping by magnetic resonance imaging nondestructively measures glomerular number and volume distribution in mice with and without nephron reduction. Kidney Int (In Press)
Clavijo-Jordan MV, Caplan MR, Bennett KM (2010) Simplified synthesis and relaxometry of magnetoferritin for magnetic resonance imaging. Magn Reson Med 64:1260–1266
Uchida M, Terashima M, Cunningham CH, Suzuki Y, Willits DA, Yang PC, Tsao PS, McConnell MV, Young MJ, Douglas T (2008) A human ferritin iron oxide nano-composite magnetic resonance contrast agent. Magn Reson Med 60:1073–1081
Clavijo Jordan MV, Beeman SC, Baldelomar EJ, Bennett KM (2014) Disruptive chemical doping in a ferritin-based iron oxide nanoparticle to decrease r2 and enhance detection with T1-weighted MRI. Contrast Media Mol Imaging 9:323–332
Danon D, Goldstein L, Marikovsky Y, Skutelsky E (1972) Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res 38:500–510
Yardeni T, Eckhaus M, Morris HD, Huizing M, Hoogstraten-Miller S (2011) Retro-orbital injections in mice. Lab Anim (NY) 40:155–160
M. Zhang, T. Wu*, and K.M. Bennett. Small blob identification in medical images using regional features from optimum scale. IEEE Trans Biomed Eng. 2015. 62(4): 1051-62
Acknowledgements
This work was funded by a grant from the NIH Diabetic Complications Consortium, The American Heart Association, The US National Institutes of Health grant NIH DK-091722, and a grant from the Hartwell Foundation.
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Bennett, K.M. et al. (2016). Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_7
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DOI: https://doi.org/10.1007/978-1-4939-3353-2_7
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