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Isolation and Propagation of Rat Peritoneal Mesothelial Cells

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Kidney Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1397))

Abstract

With the development of peritoneal dialysis in many countries, there has been much interest in the cell biology of peritoneal mesothelial cells. In this chapter we describe a reliable and reproducible method for the culture of rat primary mesothelial cells (RPMCs). This chapter outlines how to isolate mesothelial cells from rat peritoneum. The subculture of primary peritoneal mesothelial cells and the characterization by immunofluorescence is also described in detail.

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Author information

Authors and Affiliations

  1. Institute of Nephrology, The First Affiliated Hospital Sun Yat-Sen University, Guangzhou, China

    Qin Zhou & Xueqing Yu

Authors
  1. Qin Zhou
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  2. Xueqing Yu
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Corresponding author

Correspondence to Xueqing Yu .

Editor information

Editors and Affiliations

  1. Royal Hospital Melbourne Dept. Nephrology, Melbourne, Victoria, Australia

    Tim D. Hewitson

  2. Department of Nephrology, Royal Melbourne Hospital, Parkville, Melbourne, Victoria, Australia

    Edward R. Smith

  3. Department of Nephrology, Royal Melbourne Hospital, Parkville, Victoria, Australia

    Stephen G. Holt

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© 2016 Springer Science+Business Media New York

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Zhou, Q., Yu, X. (2016). Isolation and Propagation of Rat Peritoneal Mesothelial Cells. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3353-2_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3351-8

  • Online ISBN: 978-1-4939-3353-2

  • eBook Packages: Springer Protocols

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