Abstract
The renal fibroblast and phenotypically related myofibroblast are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behavior, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary human renal fibroblasts from the human kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail.
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Acknowledgments
The authors are supported by a Postgraduate Research Scholarship (SJT) and Project grant 1078694 (TDH) from the National Health and Medical Research Council of Australia (NHMRC).
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Tan, SJ., Hewitson, T.D. (2016). Propagation and Culture of Human Renal Fibroblasts. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_2
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DOI: https://doi.org/10.1007/978-1-4939-3353-2_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3351-8
Online ISBN: 978-1-4939-3353-2
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