Abstract
Imbalance between renal oxygen delivery and demand in the first hours after reperfusion is suggested to be decisive in the pathophysiological chain of events leading to ischemia-induced acute kidney injury. Here we describe blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI) for continuous monitoring of the deoxyhemoglobin-sensitive MR parameter T 2* in the renal cortex, outer medulla, and inner medulla of rats throughout renal ischemia/reperfusion (I/R). Changes during I/R are benchmarked against the effects of variations in the fraction of inspired oxygen (hypoxia, hyperoxia). This method may be useful for investigating renal blood oxygenation of rats in vivo under various experimental (patho)physiological conditions.
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Acknowledgements
This work was supported by the German Research Foundation (Deutsche Forschungsgemeinschaft, research unit: FOR 1368, grant numbers: NI 532/9-1, NI 532/9-2, SE 998/4-2). The authors wish to thank Ariane Anger, Gordana Bubalo, Kathleen Cantow, Duska Dragun, Bert Flemming, Mandy Fechner, Andrea Gerhardt, Jan Hentschel, Uwe Hoff, Mechthild Ladwig, Wolf-Hagen Schunck, Helmar Waiczies, and Sonia Waiczies for their help and support with developing the described methods.
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Pohlmann, A., Arakelyan, K., Seeliger, E., Niendorf, T. (2016). Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_10
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DOI: https://doi.org/10.1007/978-1-4939-3353-2_10
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Publisher Name: Humana Press, New York, NY
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