Abstract
Staphylococcus aureus can exist as a colonizer or can cause a spectrum of diseases. S. aureus elaborates several exotoxins and the superantigens are one among them. Staphylococcal superantigens (SSAg) cause robust activation of the immune system and acute exposure to significant amounts of SSAg can be potentially lethal. However, chronic exposure to SSAg is also possible. Administering SSAg using mini-osmotic pumps may mimic chronic recurrent exposure to SSAg. This is a relatively simple and safe way to administer purified SSAg or any other toxin/agent. In this chapter, we describe the mini-osmotic pump-mediated delivery of SSAg.
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Acknowledgements
This work was supported by National Institutes of Health Grant 5K23AR057815-02, an American College of Rheumatology Research and Education Foundation Career Development Bridge Funding Award, and a Ronald F. Kinney Executive Dean for Research Career Development Award from the Mayo Foundation (all to V.R.C.). This study was funded by NIH grants AI101172 and AI68741 (GR).
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Krogman, A.L., Chowdhary, V., Rajagopalan, G. (2016). Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_9
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DOI: https://doi.org/10.1007/978-1-4939-3344-0_9
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